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In topical studies, side effects are minimal and usually limited to mild skin irritation in sensitive individuals

Next, the microplate was placed in a SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 37 C, followed by horizontal shaking for 30 s and subsequent reading of absorbance at 570 nm every minute until the value of the most active sample surpassed that of the highest standard